分子克隆实验指南（第四版）（原版书名：Molecular Cloning: A Laboratory Manual (Fourth Edition) ） 下载 pdf 百度网盘 epub 免费 2025 电子版 mobi 在线

　　Molecular Cloning has served as the foundation of technical

expertise in labs worldwide for 30 years. No other manual has been

so popular, or so influential. Molecular Cloning, Fourth Edition,

by the celebrated founding author Joe Sambrook and new co-author,

the distinguished HHMI investigator Michael Green, preserves the

highly praised detail and clarity of previous editions and includes

specific chapters and protocols commissioned for the book from

expert practitioners at Yale, U Mass, Rockefeller University, Texas

Tech, Cold Spring Harbor Laboratory, Washington University, and

other leading institutions. The theoretical and historical

underpinnings of techniques are prominent features of the

presentation throughout, information that does much to help

trouble-shoot experimental problems.

For the fourth edition of this classic work, the content has

been entirely recast to include nucleic-acid based methods selected

as the most widely used and valuable in molecular and cellular

biology laboratories.

Core chapters from the third edition have been revised to

feature current strategies and approaches to the preparation and

cloning of nucleic acids, gene transfer, and expression analysis.

They are augmented by 12 new chapters which show how DNA, RNA, and

proteins should be prepared, evaluated, and manipulated, and how

data generation and analysis can be handled.

The new content includes methods for studying interactions

between cellular components, such as microarrays, next-generation

sequencing technologies, RNA interference, and epigenetic analysis

using DNA methylation techniques and chromatin immunoprecipitation.

To make sense of the wealth of data produced by these techniques, a

bioinformatics chapter describes the use of analytical tools for

comparing sequences of genes and proteins and identifying common

expression patterns among sets of genes.

Building on thirty years of trust, reliability, and authority,

the fourth edition of Molecular Cloning is the new gold

standard—the one indispensable molecular biology laboratory manual

and reference source.

新版特点:

? Extensive new content: 12 entirely new chapters are devoted

to the most exciting current research strategies, including

epigenetic analysis, RNA interference, genome sequencing, and

bioinformatics

? Expanded scope: the nucleic-acid-based techniques selected for

inclusion have promoted recent advances in gene transfer, protein

expression, RNA analysis, and expression of cloned genes

? Classic content: 10 original core chapters have been updated

to reflect developments and innovations in standard techniques and

to introduce new cutting-edge protocols

? Easy-to-follow format: the previous editions’ renowned

attention to detail and accuracy are fully retained

? Essential appendices: an up-to-date collection of reagents,

vectors, media, detection systems, and commonly used techniques are

included

? Expanded authorship: chapters and protocols have been

specifically commissioned from renowned experts at leading

institutions

VOLUME 1

Part 1: Essentials

1. Isolation and Quantification of DNA

2. Analysis of DNA

3. Cloning and Transformation with Plasmid Vectors

4. Gateway Recombinational Cloning

John S. Reece-Hoyes and Albertha J.M. Walhout

5. Working with Bacterial Artificial Chromosomes and Other

High-Capacity Vectors

Nathaniel Heintz and Shiaoching Gong

6. Extraction, Purification, and Analysis of RNA from Eukaryotic

Cells

7. Polymerase Chain Reaction

8. Bioinformatics

Jui-Hung Hung and Zhiping Weng

VOLUME 2

Part 2: Analysis and Manipulation of DNA and RNA

9. Quantification of DNA and RNA by Real-Time Polymerase Chain

Reaction

10. Nucleic Acid Platform Technologies

Oliver Rando

11. DNA Sequencing

Elaine Mardis and W. Richard McCombie

12. Analysis of DNA Methylation in Mammalian Cells

Paul M. Lizardi, Qin Yan, and Narendra Wajapeyee

13. Preparation of Labeled DNA, RNA, and Oligonucleotide

Probes

14. Methods for In Vitro Mutagenesis

Matteo Forloni, Alex Liu, and Narendra Wajapeyee

Part 3: Introducing Genes into Cells

15. Introducing Genes into Cultured Mammalian Cells

Priti Kumar, Arvindhan Nagarajan, and Pradeep D. Uchil

16. Introducing Genes into Mammalian Cells: Viral Vectors

Guangping Gao and Miguel Sena-Esteves

VOLUME 3

Part 4: Gene Expression

17. Analysis of Gene Regulation Using Reporter Systems

Pradeep D. Uchil, Arvindhan Nagarajan, and Priti Kumar

18. RNA Interference and Small RNA Analysis

Chengjian Li and Phillip D. Zamore

19. Expressing Cloned Genes for Protein Production, Purification,

and Analysis

Clara L. Kielkopf, William Bauer, and Ina Urbatsch

Part 5: Interaction Analysis

20. Cross-Linking Technologies for Analysis of Chromatin Structure

and Function

Tae Hoon Kim and Job Dekker

21. Mapping of In Vivo RNA-Binding Sites by UV-Cross-Linking

Immunoprecipitation (CLIP)

Jennifer C. Darnell, Aldo Mele, Ka Ying Sharon Hung, and Robert B.

Darnell

22. Gateway-Compatible Yeast One-Hybrid and Two-Hybrid Assays

John S. Reece-Hoyes and Albertha J.M. Walhout

Appendices

1. Reagents and Buffers

2. Commonly Used Techniques

3. Detection Systems

4. General Safety and Hazardous Material

　　JOSEPH SAMBROOK：

　　Sambrook was educated at the University of Liverpool (BSc (hons)

1962) and obtained his PhD at the Australian National University in

1966. He did postdoctoral research at the MRC Laboratory of

Molecular Biology (1966-67) and the Salk Institute for Biological

Studies (1967-69). In 1969 he was hired by James D. Watson to work

at the Cold Spring Harbor Laboratory in New York. Watson has been

reported to say this was the best hiring decision he ever made. Joe

was responsible for creating a combative creative environment at

CSHL that fomented discovery. Subsequently he worked at the

University of Texas Southwestern Medical Center (Dallas).

Sambrook is best known for his studies on DNA tumor viruses and

the molecular biology of normal and neoplastic cells. His Tumour

Virus Group at Cold Spring Harbor identified and mapped all of the

major genes of adenoviruses and SV40, determined their

tran*ional control in infected and transformed cells, and

elucidated the mechanism of integration of these viruses into the

genome of the host cell.[citation needed] He has also made

important contributions to the understanding of intracellular

traffic and protein folding and is an influential leader in the

field of the molecular genetics of human cancer.

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"color: #000000;">　　“In science...speed matters and

credibility matters. And compared to banking on...an anonymous

contributor in an online discussion group, you’d be better off

trusting Michael Green and Joseph Sambrook. When skimming through

the pages of Molecular Cloning, it is impossible not to gain new

insights that will push your experiments forward.”

　　 —Lab Times

媒体评论

"color: #404040;">　　“Any basic research laboratory

using molecular biology techniques will benefit from having a copy

on hand of the newly published Third Edition of Molecular Cloning:

A Laboratory Manual...the first two editions of this book have been

staples of molecular biology with a proven reputation for accuracy

and thoroughness.” —The Scientist

　　“In every kitchen there is at least one indispensable

cookbook...Molecular Cloning: A Laboratory Manual fills the same

niche in the laboratory (with) information to help both the

inexperienced and the advanced user. (It) has once again

established its primacy as the molecular laboratory manual and is

likely to be found on lab benches...around the world.” ——Trends in

Neurosciences

　　“Molecular Cloning: A Laboratory Manual has always been the

laboratory mainstay for protocols and techniques. It has a

pure-bred ancestry, and the new edition does not disappoint. (It)

includes information panels at the end of each chapter that

describe the principles behind the protocols.... The addition of

this information extends Molecular Cloning from an essential

laboratory resource into a new realm, one merging the previous

prototype with a modern molecular monograph...the next generation

of Molecular Cloning not only carries on the proud heritage of the

first two editions but also admirably expands on that tradition to

provide a truly essential laboratory manual.” —Trends in

Microbiology